OrangerV1, Main, Exploration, bibRecord, 000857

Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme

Identifieur interne : 000857 ( Main/Exploration ); précédent : 000856; suivant : 000858

Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme

Auteurs : Nethaji J. Gallage [Danemark] ; Esben H. Hansen [Danemark] ; Rubini Kannangara [Danemark] ; Carl Erik Olsen [Danemark] ; Mohammed Saddik Motawia [Danemark] ; Kirsten J Rgensen [Danemark] ; Inger Holme [Danemark] ; Kim Hebelstrup [Danemark] ; Michel Grisoni [France] ; Birger Lindberg M Ller [Danemark]

Source :

Nature Communications [ 2041-1723 ] ; 2014.

RBID : PMC:4083428

Abstract

Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4083428

DOI: 10.1038/ncomms5037
PubMed: 24941968
PubMed Central: 4083428

Affiliations:

Danemark, France

Le document en format XML

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<front><div type="abstract" xml:lang="en"><p>Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (<italic>Vp</italic>
VAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and <italic>p</italic>
-coumaric acid as demonstrated by coupled transcription/translation assays. <italic>Vp</italic>
VAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of <italic>VpVAN</italic>
 in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to <italic>Vp</italic>
VAN was identified in another vanillin-producing plant species <italic>Glechoma hederacea</italic>
 and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco.</p>
</div>
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</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000857 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000857 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4083428
   |texte=   Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:24941968" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a OrangerV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

Serveur d'exploration sur l'oranger

Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme

Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.